| JD005 화합물이 인간치수유래줄기세포의 상아질 모세포 분화에 미치는 영향에 관한 연구 |
김다솔1 
, 이남걸2
, 장혜옥1
, 안진희3
, 김형룡4
|
1부산대학교 치의학전문대학원 치과약리학교실, 치의생명과학연구소
2제이디바이오사이언스, 기업부설연구소
3광주과학기술원, 화학과
4전북대학교 치과대학 약리학교실 |
| The effect of JD005 compound on odontogenic differentiation of human dental pulp stem cells |
|
Da-Sol Kim1
, Nam-Geol Lee2
, Hye-Ock Jang1
, Jin-Hee Ahn3
, Hyung-Ryong Kim4
|
1Dental Pharmacology, School of Dentistry, Pusan National University
2Research and Development Center, JD Bioscience
3Department of Chemistry, Gwangju Institute of Science and Technology
4Department of Pharmacology, College of Dentistry, Jeonbuk National University |
Correspondence:
Hye-Ock Jang, Tel: 82-51-510-8236, Fax: 82-51-510-8233,
Email: jho9612@pusan.ac.kr
Jin-Hee Ahn, Tel: 82-62-715-4621, Fax: 82-62-715-3609,
Email: jhahn@gist.ac.kr
Hyung-Ryong Kim, Tel: +82-63-270-4024, Fax: +82-63-270-4024,
Email: hrkimdp@gmail.com
|
|
Received: 10 June 2021 • Revised: 12 August 2021 • Accepted: 19 August 2021 |
| Abstract |
|
Preservation of natural teeth and their structure is critical for chewing, swallowing, and better speech. Dentin and dental tissues are crucial in maintaining natural teeth and their structure, but treatment of dental tissue and dentin fracture is chal-lenging for dentistry. Hence, scientific understanding needs to be translated to discover novel dental treatments. Dental pulp stem cells (DPSCs) are mesenchymal stem cells (MSCs) isolated from the third molar pulp. These cells have similar characteristics to MSCs with a unique property where DPSCs can differentiate into dentin forming odontoblast-like cells. Here, we made an effort to evaluate the potential substrate that induces odontoblastic differentiation of DPSCs. In this study, JD005, a modified A2A antagonist from ZM-241385, was determined to enhance OD. Specifically, JD005 treatment signifi-cantly enhanced the OD markers like alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2), OPN, dentin sialophosphoprotein (DSPP), and dentin matrix protein 1 (DMP1). Additionally, the DPSCs treated with JD005 showed enhanced SMAD phosphorylation associated with differentiation. These observations distinctively suggest that JD005 po-tentially contributes to dentin regeneration via odontoblast differentiation. |
| Key Words:
DPSCs; JD005; dentine regeneration; odontoblasts; RUNX2; DSPP; SMAD phosphorylation |
|
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