Antibacterial effect of Zingiberaceae extracts mediated photodynamic therapy on Streptococcus mutans

Hwang,  Hye-Rim; Kang,  Si-Mook; Lee,  Eun-song; Kim,  Baek-Il

J Korean Dent Assoc > Volume 57(10); 2019 > Article

The Journal of The Korean Dental Association 2019;57(10):560-568.
Published online October 31, 2019.

Streptococcus mutans에 대한 생강과 천연추출물의 광역학 항균효과

황혜림¹, 강시묵², 이은송³, 김백일⁴

^¹연세대학교 치과대학 예방치과학교실, BK²¹ 플러스 통합구강생명과학사업단
^²연세대학교 치과대학 예방치과학교실, BK²¹ 플러스 통합구강생명과학사업단
^³연세대학교 치과대학 예방치과학교실, BK²¹ 플러스 통합구강생명과학사업단
^⁴연세대학교 치과대학 예방치과학교실, BK²¹ 플러스 통합구강생명과학사업단

Antibacterial effect of Zingiberaceae extracts mediated photodynamic therapy on Streptococcus mutans

Hye-Rim Hwang¹, Si-Mook Kang², Eun-song Lee³, Baek-Il Kim⁴

¹Department of Preventive dentistry & Public Oral Health, BK 21 PLUS Project, Yonsei University College of Dentistry
²Department of Preventive dentistry & Public Oral Health, BK 21 PLUS Project, Yonsei University College of Dentistry
³Department of Preventive dentistry & Public Oral Health, BK 21 PLUS Project, Yonsei University College of Dentistry
⁴Department of Preventive dentistry & Public Oral Health, BK 21 PLUS Project, Yonsei University College of Dentistry

Abstract

Purpose
This study evaluated the antibacterial effects of curcuma, ginger, and finger root extracts in water-soluble powder on planktonic Streptococcus mutans(S.mutans), which is known to cause dental caries, in order to confirm whether these extracts could perform as photosensitizers for the effects of photodynamic therapy (PDT). Methods: This study used the strain of streptococcus mutans ATCC 25175 distributed by the Korean Collection for Type Cultures of the Korea Research Institute of Bioscience & Biotechnology. Commercial edible curcuma, ginger and finger root were used as the natural extracts for the use of photosensitizer. To extract organic solvent, 3 g of each powder was mixed in 30mell of dimethyl sulfoxide (DMSO, VWR, Germany) before extraction. 1.8mell of the photosensitizer solution, manufactured in the concentrations of 5, 0.5, and 0.05mg/mell, was mixed with 0.2mell of the S. mutans culture medium that had been cultured for 2 days. To induce the photodynamic reaction, Qraycam (AIOBIO, Seoul, Korea) equipped with 405 nm LED was used to expose light for 5 minutes to irradiate 59 nW energy for 300 seconds. Results: Compared with the case with no light, a higher photodynamic therapeutic effect was confirmed with 0.05mg/mell curcuma powder extract, the concentration of 0.5mg/mell and LED light of 405 nm wavelength (p=0.000, p=0.003). 0.05mg/mell of curcuma powder extract and the concentration of 0.5mg/mell showed 100% antibacterial effect when exposed to light, whereas the concentration of 5mg/mell showed 11.95% antibacterial effect. When exposed to light, 0.05mg/mell of ginger powder extract showed an antibacterial effect which didn't statistically decrease. The concentrations of 0.5mg/mell and 5mg/mell did not show any antibacterial effects. As a result of examining any photodynamic therapeutic effects of finger root powder extract on S. mutans, no statistically significant effect was found. Conclusion: The curcuma powder extract is expected to perform as a photosensitizer. Even though belonging to the same ginger family, ginger powder and finger root powder seem difficult to perform as photosensitizer.

Key Words: photodynamic therapy;natural photosensitizer;Quantitative Light-induced fluorescence;