| Zoledronate이 UMR-106 세포의 증식과 조골세포 형성에 미치는 영향 |
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정기훈1, 류동목2, 지유진3, 이덕원4, 이현우5 |
1경희대학교 일반대학원 치의학과 구강악안면외과학
2경희대학교 치의학전문대학원 구강악안면외과학교실
3경희대학교 치의학전문대학원 구강악안면외과학교실
4경희대학교 일반대학원 치의학과 구강악안면외과학
5경희대학교 치의학전문대학원 치과약리학교실 |
| Zoledronate(Zometa(R))inhibits the formation of osteoblast in rat osteoblastic cell line UMR-106 |
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Ki-Hoon Jeong1, Dong-Mok Ryu2, Yu-Jin Jee3, Deok-Won Lee4, Hyun-Woo Lee5 |
1Dept. of Oral and Maxillofacial Surgery, Graduate School of Denistry, Kyunghee University
2Dept. of Oral & Maxillofacial Surgery, Professional Graduate School of Denistry, Kyunghee University
3Dept. of Oral & Maxillofacial Surgery, Professional Graduate School of Denistry, Kyunghee University
4Dept. of Oral and Maxillofacial Surgery, Graduate School of Denistry, Kyunghee University
5Dept. of Dental Pharmacology, Professional Graduate School of Denistry, Kyunghee University |
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| Abstract |
Purpose
: The purpose of this study is to identify the effect of zoledronate(Zometa(R)), which is most common nitrogen containing bisphosphonate, on survival, proliferation, and differentiation of osteoblast. Material & Methods: Twenty four cell culture plates containing essential medium were seeded with UMR-106 cell lines, at density of 5 x 104 cells per plates. Each plates were incubated with 5% CO2 incubator at 37°C. Starting from 2 days after incubation, cell culture medias were replaced, and added with osteogenesis induction media and 0, 0.01, 0.1, 0.5, 1, 3muM of zoledronate(Zometa(R)), every 2 days, for 12 days. Control group was plates not added with zoledronate(0muM), and experiment group were plates added with different concentration of zoledronates(0, 0.01, 0.1, 0.5, 1, 3muM). Mature osteoblasts were identified with Alizarine Red staining, and protein samples were collected. Optical density was determined at wavelength of 405nm with ELISA reader. For viability analysis, cells were harvested and incubated with propidium iodide, and analysed with flow cytometry. Western blot technique was used to analyse Runx2 protein of osteoblast. Results : Secretion of bone matrix decreased as zoledronate concentration increased, and zoledronate did not effect survival rate of UMR-106 cells when measured with flow cytometer. Expression of Runx2 protein was inhibited as zoledronate concentration increased. Conclusion : From the results, we were able to identify that increase of zoledronate concentration inhibited differentiation of UMR-106 cell to osteoblast, without effecting quantity or survival rate. |
| Key Words:
ZoledronateUMR-106OsteoblastRunx2;;;;; |
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