| Simvastatin이 UMR-106 세포의 조골세포 형성에 미치는 영향 |
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황의관1, 류동목2, 지유진3, 이덕원4, 이현우5 |
1경희대학교 일반대학원 치의학과 구강악안면외과학
2경희대학교 치의학전문대학원 구강악안면외과학교실
3경희대학교 치의학전문대학원 구강악안면외과학교실
4경희대학교 일반대학원 치의학과 구강악안면외과학
5경희대학교 치의학전문대학원 치과약리학교실 |
| Effects of Simvastatin on osteogenesis of rat osteoblast-like cells, UMP-106 |
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Eui-Kwan Hwang1, Dong-Mok Ryu2, Yu-Jin Jee3, Deok-Won Lee4, Hyun-Woo Lee5 |
1Dept. of Oral and Maxillofacial Surgery, Graduate School of Denistry, Kyunghee University
2Dept. of Oral & Maxillofacial Surgery, Professional Graduate School of Denistry, Kyunghee University
3Dept. of Oral & Maxillofacial Surgery, Professional Graduate School of Denistry, Kyunghee University
4Dept. of Oral and Maxillofacial Surgery, Graduate School of Denistry, Kyunghee University
5Dept. of Dental Pharmacology, Professional Graduate School of Denistry, Kyunghee University |
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| Abstract |
Purpose
The purpose of this study is to investigate the effects of Simvastain, which is HMG-CoA reductase inhibitor, on proliferation and differentiation of osteoblast. Materials & Methods : Twenty-four cell culture plates containing essential medium were seeded with UMR-106 cell lines, at density of 5 x 104 cells per plate. Each plates were incubated with 5% CO2incubator 37°C. Starting from 2 days after incubation, cell culture medias were replaced with Osteogenesis induction media every 2 days, for 12 days. In some plates, 0.01, 0.1, 1, 10, 100muM of Simvastatin were added with Osteogenesis induction media, and classified as "test group". Those not added with Simvastatin were classified as "control group". Results : 1. When Alrizarin Red staining was observed with naked eye, control group showed normal deep red color, but test group show rapid decrease of red color as Simvastatin concentration increased more than 0.1muM. 2, When observed with microscope, compared to control group, amount of osteo matrix stained with Alrizarin Red decreased rapidly in Simvastatin concentration more than 0.1muM. 3. In optical density analysis, regarding control group as a basis, mineral deposition decreased rapidly when Simvastatin concentration increased more than 0.1muM. 4. In flow cytometry analysis, survival rate of UMR-106 cell showed no changes in both control group and test group. Conclusion : From the above results, we were able to identify that Simvastatin inhibited osteogenesis without effecting survival or cell number of osteoblasts. |
| Key Words:
Osteogenesis, Simvastatin, UMR-106;;;;; |
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