Altered homeostatic factor of db/db mice’s periodontal ligament: A pilot study

Choi, Wonjun; Xiong, Ni; Lim, Won Hee

doi:2023.61.7.460

J Korean Dent Assoc > Volume 61(7); 2023 > Article

ORIGINAL ARTICLE

The Journal of The Korean Dental Association 2023;61(7):460-468.
DOI: https://doi.org/10.22974/jkda.2023.61.7.002 Published online June 30, 2023.

제2형 당뇨모델 생쥐에서 치주인대조직 내항상성조절인자 변화: 예비연구

최원준

, Ni Xiong

, 임원희

서울대학교 치의학전문대학원 치과교정학교실

Altered homeostatic factor of db/db mice’s periodontal ligament: A pilot study

Wonjun Choi

, Ni Xiong

, Won Hee Lim

Department of Orthodontics, Seoul National University Dental Hospital

Correspondence: Won Hee Lim, Tel: +82 2 2072 0272, Fax: +82 2 2072 3817,
Email: whlim@snu.ac.kr

Received: 11 April 2023 • Revised: 14 April 2023 • Accepted: 16 June 2023

Abstract

Purpose
Diabetes is a risk factor for periodontal disease and can cause alveolar bone loss. The purpose of this study was to explore the effect of diabetes on homeostatic factor of periodontal tissue using leptin receptor (db/db) mice.
Materials and Methods
The db/db mice(experimental group) were compared with the wild type mice (control group) using histology and immunohistochemical analyses. Collagen fibers were evaluated using H&E, picrosirius red, fibromodu-lin staining and osteogenic factors (periostin, osteopontin, osteocalcin, osterix), and factors related to osteoclasts (OPG, RANKL) in the PDL were investigated.
Results
Collagen fibers and osteogenic factors in the PDL showed no differences between a control and an experimental group. In an experimental group, expression of receptor activator of nuclear factor kappa-Β ligand (RANKL) was similar compared with a control group, while expression of osteoprotegerin (OPG) was decreased.
Conclusions
RANKL/OPG ratio may be one of homeostatic factors related to bone remodeling in diabetic mice.

Key Words: diabetes; homeostasis; periodontal tissue; RANKL; OPG