Combined effects of a chemically cross-linked porcine collagen membrane and highly soluble biphasic calcium phosphate on localized bone regeneration

Kim,  You-Kyoung; An,  Yin-Zhe; Cha,  Jae-Kook; Lee,  Jung-Seok; Jung,  Ui-Won; Choi,  Seong-Ho

doi:2018.56.12.667

J Korean Dent Assoc > Volume 56(12); 2018 > Article

The Journal of The Korean Dental Association 2018;56(12):667-685.
DOI: https://doi.org/10.22974/jkda.2018.56.12.002 Published online December 31, 2018.

Combined effects of a chemically cross-linked porcine collagen membrane and highly soluble biphasic calcium phosphate on localized bone regeneration

You-Kyoung Kim¹, Yin-Zhe An², Jae-Kook Cha³, Jung-Seok Lee⁴, Ui-Won Jung⁵, Seong-Ho Choi⁶

¹Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry
²Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry
³Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry
⁴Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry
⁵Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry
⁶Department of Periodontology, Research Institute for Periodontal Regeneration, Yonsei University College of Dentistry

Abstract

Objectives
Aim of this study was to evaluate bone regenerative efficacy of a chemically cross-linked porcine collagen membrane (CM) when used in combination with highly soluble biphasic calcium phosphate (BCP). Materials and methods: Physiochemical properties of the experimental collagen membrane were analyzed. Four circumferential defects with diameter of 8 mm were created in each calvarium of New Zealand white rabbits (n = 10). Defects were randomly allocated to one of following 4 groups: 1) BCP-CM (BCP (20% hydroxyapatite/80% beta-tricalcium phosphate) covered with the prepared collagen membrane), 2) BCP (only BCP used), 3) CM (only the prepared collagen membrane used), and 4) C (control; only blood clot). After 2 weeks (n = 5) and 8 weeks (n = 5), histologic and histomorphometric analyses were performed. Results: The experimental collagen membrane exhibited dense and compact structure, relatively high tensile strength and lower degradability. Histologic analyses revealed that new bone increased rapidly at 2 weeks, while defect was preserved at 8 weeks. Histomorphometric analyses revealed that the new bone areas increased in the BCP-grafted groups over 8 weeks, with BCP-CM exhibiting greater total augmented area than that of BCP group both at 2 weeks (27.12pm3.99 versus 21.97pm2.27mm2) and 8 weeks (25.75pm1.82 versus 22.48pm1.10mm2) (P < 0.05). Conclusions: The experimental collagen membrane successfully preserved localized defect for 8 weeks despite early rapid resorption of BCP. Within the study limitations, combined use of the chemically cross-linked porcine collagen membrane and highly soluble BCP aided localized bone regeneration.

Key Words: Biodegradation;Biphasic calcium phosphate;Bone regeneration;Bone substitute;Collagen membrane;