Comparison of Salivary Stress Hormone Levels between Periodontitis Patients and Healthy Subjects in Korea

Lee,  Sol; Heo,  Narae; Heo,  Seok-Mo

J Korean Dent Assoc > Volume 54(11); 2016 > Article

The Journal of The Korean Dental Association 2016;54(11):897-906.
Published online November 30, 2016.

Comparison of Salivary Stress Hormone Levels between Periodontitis Patients and Healthy Subjects in Korea

¹Department of Dental Pharmacology, School of Dentistry, Chonbuk National University
²Department of Nursing, Hansei University
³Department of Periodontology, School of Dentistry, Chonbuk National University

Abstract

Objectives
Periodontitis is multifactorial disease mainly caused by microbial community. Recently, some research has been conducted to find other possible risk factors including stress hormones related to periodontitis. Psychological stress can affect the periodontal health by a variety of biological mechanisms. This study compared the stress hormone levels in healthy subjects and patients with periodontal disease using saliva in order to investigate the association between periodontitis and stress. Methods: The human saliva was collected from 38 periodontally healthy individuals and 34 patients with chronic periodontitis under Institutional Review Board. Their age was 20-60 years (40.3pm10.45). From these samples, determination of salivary levels of cortisol and Dehydroepiandrosterone (DHEA) performed by enzyme immunoassay kit (Salimetrics Europe, Suffolk, UK). The independent t-test and Mann-Whitney test for trend was applied using IBM SPSS statistics version 12.0 Program to analyze statistically significant differences. Results: Salivary cortisol levels of periodontitis patients were higher than those levels of healthy subjects (P < 0.001), while salivary DHEA levels of periodontitis patients were not significantly different (P = 0.431). Salivary cortisol/DHEA ratio of periodontitis patients was higher than those levels of healthy subjects (P < 0.001). Conclusions: Our study demonstrates the high levels of cortisol concentrations and cortisol/DHEA ratio in saliva of periodontitis patients than those of healthy subjects. Since cortisol levels and cortisol/DHEA ratio can be significant factors related to the severity of periodontal disease, our study would be helpful for early diagnosis and treatment of periodontal disease.

Key Words: Periodontitis;Saliva;Stress hormone;ELISA;Cortisol;Dehydroepiandrosterone (DHEA);