| 결절형 Tawa육종의 Collagenase에 관한 연구 |
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정태영1, 신철야2, 다화민일3 |
1서울대학교 치과대학 생화학교실
2오사카치과대학 생화학교실
3오사카치과대학 생화학교실 |
| Collagenolytic Activity of Solid Tawa Sarcoma |
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Tai-Young Chung1, Tetsuya Sakaki2, Toshikazu Tawa3 |
1Dept. of Biochemistry College of Dentistry S.N.U.
2Dept. of Biochemistry Osaka Dental University
3Dept. of Biochemistry Osaka Dental University |
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| Abstract |
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True collagenolytic enzymes in animal tissues were first demonstrated by Gross and Lapiere (1962), who showed the ability of such an enzyme in the culture medium of living explants of tadpole tissue to degrade a specific substrate of undenatured collagen under physiological conditions. Recently, tumor-associated collagenolytic activity has been demonstrated in human neoplasm and in ascites V Carcinoma. This investigation have been peforme to determine whether or not a collagen lytic enzyme could e found in isolated solid Tawa sarcoma of Donryu female rat obtained the culture medium. The results were as follows.
1. 11.5mg% of hydroxyproline contained in Donryu rat skin collagen, which was extracted by 0.5M acetic acid.
2. Cultivation of solid Tawa sarcoma tissues on reconstituted rat skin collagen gels showed lysis of adjacent gel after 18 hours, and much more extensive lysis after 5 days.
3. Collagen substrate was not attacked by the common proteolytic enzymes, trypsin, pepsin, and pronase. |
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