| 미뢰함유설상피와 비함유설상피 단백질에 관한 SDS-PAGE적 비교 연구 |
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김현만1, 황성명2, 고재승3, 김정근4 |
1서울대학교 치과대학 및 치학연구소 구강해부학교실
2서울대학교 치과대학 및 치학연구소 구강해부학교실
3서울대학교 치과대학 및 치학연구소 구강해부학교실
4서울대학교 치과대학 및 치학연구소 구강생화학교실 |
| SDS-PAGE OF THE LINGUAL EPITHELIUM WITH SPECIAL REFERENCE TO TASTE BUDS |
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Hyun-Man Kim1, Sung-Myung Hwang2, Jae-Seung Ko3, Jung-Keun Kim4 |
1Department of Oral Anatomy College of Dentistry and Dental Research Institute, Seoul NationalUniversity
2Department of Oral Anatomy College of Dentistry and Dental Research Institute, Seoul NationalUniversity
3Department of Oral Anatomy College of Dentistry and Dental Research Institute, Seoul NationalUniversity
4Department of Oral Biochemistry College of Dentistry and Dental Research Institute, SeoulNational University |
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| Abstract |
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As a study to elucidate whether taste buds contain specific proteins, rat dorsal lingual epithelium was analysed by electrophoresis. The epithelium of the vallate papilla (with numerous taste buds), the area of the fungiform papilla 9with a few taste buds), and the area between vallate papilla and large filiform papilla (not containing taste buds) were strippled off by treatment with 0.7% EDTA. The epithelial protein was extracted by 1% SDS and 1% Mercaptoethanol in 0.01M phosphate buffer (pH7.2). Extracts were analysed by disc SDS-PAGE. Because the patterns of protein composition from each site were similar with each other as a whole, it is concluded that taste buds do not contain specific protein detected by SDS-PAGE in adult rat. But a protein on M.W. 49000 which lies in the area of molecular weight of keratin molecules was found only in the epithelium containing taste buds. This results suggested that the epithelium containing taste buds differentiate dissimilarly to the epithelium not containing taste buds. |
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