| Enzyme-linked immunosorbent assay를 이용한 국소적, 유년성 치주염 환자의 혈청내 Actinobacillus actinomycetemcomitans Y4 균주 항체역가에 관한 연구 |
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정종평1, 정진형2, 최선진3 |
1서울대학교 치과대학 치주과학 교실
2단국대학교 치과대학 치주과학 교실
3서울대학교 치과대학 미생물학 교실 |
| Enzyme-Linked Immunosorbent Assay for the detection of serum lgG and lgM Antibodies to Actinobacillus Actinomycetemcomitans Y4 in Localized Juvenile Periodontitis |
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Jong-Pyeong Jeong1, Jin-Hyeong Jeong2, Seon-Jin Choe3 |
1Dept. Periodontology, School of Dentistry. S.N.U.
2Dept. Periodontology, School of Dentistry, Dan Kuk University
3Dept. Microbiology, School of Dentistry. S.N.U. |
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| Abstract |
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Twelve patients of localized juvenile periodontitis were evaluated to detection of serum IgG and IgM antibodies to Actinobacillus actinomycetemcomitans Y4 strain. Sera were isolated from those patients. Antibody titer of patients sera to Aa stran Y4 strain. Sera were isolated from those patients. Antibody titer of patients sera to Aa stran Y4 were determined by modified enzyme-linked immunosorbent assey (ELISA) using sonicated and formalin-fixed whole Aa y4 strain for detection of serum IgG and IgM antibody titers. To compare with health control and L.J.P., we used 12 healthy dental student who did not exhibited any gingivits. Results were determined by using ELISA reader at 400mm absorbance value. Data analysis were performed with comparison of the regression functions relating absorbance to dilution and Dunnett t-test. Significant high antibody titer to As Y4 in L.J.P. sera were shown in this examination(281. 4 Eu-G to 162.80 Eu-G, 106.0 Eu-M to 40.0 Eu-M for sonicated As Y4 antigen and 653.960. to 138.117 Eu-M for intact As Y4) and this data were also statistically significant (P<0.05). This work was supported in part from Seoul national University Hospital Grant and Korean Science Foundation. |
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